Quickest 2h 41min
There are 2 train companies operating 5 routes - TER 3, FR-830, TGV Lyria, TGV 505, TER 2, 1 bus from Besançon to Lyon. The cheapest train costs $46 and is 3h 46min.
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There are 3 ways to travel from Besançon to Lyon. 2 trains, 1 bus and 1 by car are possible between the two areas. The by car from Besançon to Lyon is the cheapest. The train from Morre to Lyon is the fastest. The train operated by running from Morre to Lyon is the most convenient.
It's important to check each mode when booking your ticket so that you can find the best method for you. The cost of each type varies differently, so be sure to use Faretrotter to keep an eye out!